Exon and Gene Level Expression Profiling of Islets in Gaz Knockout Mice on Control and High Fat Diets

Transcriptome analysis of RNA samples from islets To gain grater insight into the role and mechanisms behind the inhibitory G-Protein, Gaz we used global gene expression profiling. In mice, transgenic loss of B-cell Gaz is protective against high fat diets. through increased insulin secretion. This mechansism is poorly understood and here, using global transcriptomics we attempt to understand the genetic impact of Gaz knockout on control and HFD diets in male mice. Raw probe cell intesity data was analyzed at both the gene- and exon-level using the Robust Multichip Analysis (RMA) algorithm within the Affymetrix Expression Console 1.3 software package