scRNA sequencing time series of human iPSC-derived hematopoietic progenitors (with or without Notch activation from day 0-2) developing into T cells from day 12 to day 42 compared with freshly isolated human thymocytes.

scRNA sequencing time course covering 5 time points from progenitors to maturing T cells Overall design: We undertook scRNA sequencing of day 12 progenitors with and without Notch activation from day 0-2, day 13 (one day of co-culture), day 16 (early events toward the T/NK lineage), day 20 (large populations of T/NK progenitors seen by flow at this time point), and day 42 (maturing T cells). N=2 for each time point from separate differentiations. 1 sample of freshly isolated post natal human thymocytes was used as a primary cell control. All samples were FACS sorted for live, CD45+ cells before single cell capture using InDrops technique at HMS.