Whole genome analysis of 12 Pandoraea species isolates from cystic fibrosis patients. PandoraeaWGS

Aim. Genetic characterization of Pandoraea isolates recovered from cystic fibrosis patients. Materials & methods. The whole genome sequence of 12 Pandoraea strains was determined using Illumina technology. The position of the strains within the genus Pandoraea was analyzed using selected partial gene sequences, core genome Multi-locus Sequence Typing and Average Nucleotide Identity analysis. Furthermore, the sequences were annotated. Results. The results show that some strains previously identified as Pandoraea pnomenusa, Pandoraea sputorum, Pandoraea oxalativorans, and Pandoraea pulmonicola belong to novel species. The strains did not harbor acquired antibiotic resistance genes but encoded an OXA-type ß-lactamase. No virulence genes were identified. Conclusion. The taxonomy of the genus Pandoraea needs to be revised.