Dataset for "Projection-TAGs Enable Multiplex Projection Tracing and Multi-modal Profiling of Projection Neurons"

Projection-TAGs is a retrograde AAV platform that allows multiplex tagging of projection neurons using RNA barcodes. The dataset contains the sequencing data and analysis meta data, as well as R scripts, that are required to reproduce the snRNA-seq analysis and combinatorial snRNA-seq and snATAC-seq analysis related to Fig 2 (a multi-modal single-cell atlas of mouse cortex), Fig 3 (Projection-TAGs revealed axonal collaterals and complex wiring diagram), Fig 4 (Genomic regulatory networks of cortical cell types using Projection-TAGs), and Fig 5 (Projection-TAGs enable the detection of projection neurons acutely activated by visceral pain) of the manuscript.