Expression and transcriptional regulation dynamics of hepatic lincRNAs

Gene expression in adult male and female mouse liver was assayed by RNA-seq, as part of a study on hepatic lincRNAs. Total liver RNA was prepared from 12 individual male and 12 individual female mice. Four independent pools, comprised of RNA isolated from 6 individual male or female livers (2 pooled biological replicates for each sex) were then prepared and used for RNA-seq.本研究以肝脏基因间长链非编码RNA（hepatic lincRNAs）为研究对象，采用RNA测序（RNA-seq）技术检测成年雌雄小鼠肝脏的基因表达水平。实验从12只雄性小鼠与12只雌性小鼠体内分别提取肝脏总RNA；随后构建4个独立混合样本池：每个性别各取6只个体的肝脏RNA进行混合，且每个性别设置2个生物学重复混合样本，最终将全部混合样本用于RNA-seq实验。