Bulk RNA-sequencing of cell types isolated by FACS from normal human prostates

Differential expression of prostatic cell types were determined using RNA-sequencing of FACS sorted cell types from normal human prostates. The cell types sequenced were basal epithelia, luminal epithelia, a heterogeneous fibromuscular stroma and a poorly understood "other" epithelial population. Fresh human prostate specimens were digested into single-cell suspensions for flow cytometry, populations of cells were subject to bulk RNA-sequencing.