Frequency of perithecia without neck in crosses homozygous for the Δpah mutations.

aEach cross was performed in duplicate on two separate Petri dishes inoculated with mat+ and mat− strains. When the mycelia were confluent, sterile water was poured to disperse microconidia over the surface of the cultures and promote fertilization of female organs of the compatible mating-type. 5 to 9 independent counts were made seven days after fertilization on 150 to 400 perithecia formed on different sectors of the two dishes. Data from one*, two** or three*** assays were pooled. Δpah2 Δpah4, Δpah2 Δpah6 and Δpah2 Δpah7 had the same % of perithecia without neck as Δpah2. All the perithecia from Δpah1 Δpah5, Δpah2 Δpah5, Δpah3 Δpah5, Δpah4 Δpah5 and Δpah6 Δpah5 had no neck. All the perithecia from the other double mutants had a beak.