Genome-wide identification of target genes for the histone demethylase Phf8 in the oligodendroglial cell line CG4 [Phf8OE]

To identify target genes of the histone demethylase Phf8 in oligodendroglial cells in a genome-wide and unbiased approach, RNA-sequencing studies were performed on CG4 cells transduced with control or Phf8-overexpressing retroviruses. Transduced, GFP-expressing cells underwent fluorescence-activated cell-sorting, were expanded and then differentiated for three days. Two biological replicates were used for each condition. Gene expression analysis revealed an increase in oligodendrocyte progenitor proliferation and a general decrease in cell differentiation.为在全基因组范围内采用无偏倚研究策略鉴定少突胶质细胞内组蛋白去甲基化酶Phf8的靶基因，研究人员对转导了对照或Phf8过表达逆转录病毒的CG4细胞开展了RNA测序（RNA-sequencing）实验。转导后表达绿色荧光蛋白（GFP）的细胞经荧光激活细胞分选（fluorescence-activated cell-sorting）分离，经扩增后诱导分化三天。每个实验条件均设置两个生物学重复。基因表达分析结果显示，少突胶质细胞祖细胞的增殖水平升高，细胞分化程度普遍降低。