GC-MS data

The GC–MS, and GC-FID runs were carried out on
a two parallel GC-QP2010 and GC2010 instruments (Shimadzu, Kyoto, Japan). The
GC column used was a 30 m × 0.25 mm i.d. × 0.25 μm df Supelcowax-10 column
(MilliporeSigma). Helium was exploited as carrier gas, at a constant linear
velocity of 30.0 cm/s which corresponding to an inlet pressure of 26.4 kPa for
GC-MS and 97.4 kPa for GC-FID. The temperature program was the same in both
analysis-type: 40°C at 3° C/min to 250°C, at 10°C/min to 280°C, held 10 min.

GC-MS ion source temperature was set at 200°C;
the interface temperature, 250°C. Scan range was set to m/z 40–360, with a
scanning rate of 2000 amu/s. FFSNC 3.0 (Shimadzu) and NIST11 (Wiley) commercial
libraries were used for identification, applying two filters, namely a spectrum
similarity match over 85% and Linear Retention Index (LRI) (related to a C4-C24
FAMEs mixture) agreement in the ±15 range.

The FID temperature was set at 280°C (sampling
rate 40 ms) and gas flows were 40 mL/min for hydrogen and 400 mL/min for air,
respectively.

The data handling was supported by
GCMSsolution ver.4.30 and GCsolution software (Shimadzu) for GC-MS and GC-FID
analysis, respectively.