List of the 30 U-box genes identified in C. reinhardtii and their sequence characteristics.

aF and R represent the forward and reverse directions on the chromosome, respectively.bWoLF PSORT. N, nucleus; C, chloroplast; c, cytoplasm; V, vacuole; E, endoplasmic reticulum; M, mitochondria; n.a., not available.In total, 30 CrPUB proteins were obtained by BLASTP search using the C. reinhardtii V5.5 proteome database and PUB proteins from Arabidopsis thaliana and Oryza sativa as queries. The 30 CrPUB genes were named based on their chromosome position. The molecular weights and pIs of the 30 CrPUB proteins were predicted using ExPASy. The CrPUB sub-cellular locations were predicted using the WOLF PSORT program.List of the 30 U-box genes identified in C. reinhardtii and their sequence characteristics.