Primers used for in vitro dsRNA synthesis.
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aUnderlined nucleotide sequence is the T7 RNA polymerase promoter sequence. A pair of gene specific primers with the T7 promoter sequence at the 5′ ends of both primers was used to amplify the desired templates. Amplified PCR products were used as templates for in vitro dsRNA synthesis.bItalic nucleotide sequence is the Not 1 restriction enzyme recognition sequence.
创建时间：
2015-12-02
