Isoginkgetin induces autophagic cell death in hepatocellular carcinoma [ChIP-seq 2]

Purpose: Our previous studies have identified isoginkgetin as an inducer of autophagic cell death in hepatocellular carcinoma. We set out to analyze how isoginkgetin regulates the genome-wide enhancer activity in HepG2 cells. Methods: ChIP-seqs for H3K4me, H4K8ac, H3K27me3 and H3K9ac were performed in HepG2 followed by treatment with 20 μM isoginkgetin for 24 h. Conclusions: Isoginkgetin treatment reduces the expression of glucose transporters and inhibts SLC2A1 enhancer activity in HepG2 cells. ChIP-seq was performed in HepG2 cells followed by treatment with 20 μM Isoginkgetin for 24 h. The genome-wide enhancer activity was studied by H3K4me, H4K8ac, H3K27me3 and H3K9ac ChIP sequencing.