UMI-4C in wild-type and p63-null surface ectoderm cells. UMI-4C in wild-type and p63-null surface ectoderm cells

Purpose: Validate HiChIP-identified long-range chromatin interactions at the TFAP2C locus, in a viewpoint-specific manner. Methods: UMI-4C libraries targeting 3 individual viewpoints at the TFAP2C locus were generated in duplicate, in surface ectoderm cells with and without p63. Replicates were sequenced on Illumina NextSeq sequencer, and reads were aligned and filtered for valid long-range interactions using HiCPro. Results: Dynamic long-range interactions at the TFAP2C locus were detected with and without p63 in surface ectoderm cells. Conclusion: Long-range interactions detected via HiChIP were confirmed in the surface ectoderm by this technique, validating the HiChIP method through an orthogonal approach. Overall design: UMI-4C libraries, generated using DpnII digestion and TFAP2C locus primer viewpoints, were sequenced in duplicate on Illumina NextSeq sequencer.