The results of RNA-seq for EhracM gene silencing strain and EhracJ gene silencing strain
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To know the role of Rho small GTPases of enteric protozoan parasite Entamoeba histolytica, ten Rho small GTPases were specifically silenced and examined the effect on virulence-related phenotypes. Among them, EhRacM gene silence caused an enhancement in macropinocytosis. To clarify transcriptomic difference in EhRacM gene silenceing strain, RNA-seq analysis was conducted. Overall design: To know the role of Rho small GTPases of enteric protozoan parasite Entamoeba histolytica, ten Rho small GTPases were specifically silenced and examined the effect on virulence-related phenotypes. Among them, EhracM gene silencing caused an enhancement in macropinocytosis. Also, EhracJ gene silencing caused a slight decrease in trogocytosis. To clarify the transcriptomic differences in EhracM gene silenced strain and EhracJ gene silenced strain, RNA-seq analysis was conducted.
