Evolutionarily conserved host factors required for Brucella abortus (S2308) infection in both Drosophila S2 and mammalian cell lines
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aColony forming units (CFUs) of the untreated control was normalized as 100%.bData represent the means��standard deviations from at least three independent experiments.**represents significant at PcDominant-negative mutant.dNot detected.eData resulted from image analysis using NIH Image J software describe in Materials and Methods.fNo accurate data, ��30% of infected cells with replicating bacteria.gMEFs are deficient in class IA PI3Ks p85�� and p85�� (p85��?/?p85��?/?).hDouble strand RNA targeting to the two PI3Ks Pi3K21B (CG2699) and Pi3K92E (CG4141) in class IA and IB, respectively.iDouble strand RNA targeting to all the four members of PI3Ks [Pi3K21B, Pi3K92E, Pi3K68D (CG11621, class II PI3K) and Pi3K59F (CG5373, class III PI3K)] in the fly genome.
