Leaf proteome of Glycine max in response to ethylene, ABA and combined ABA+ethylene treatments

Phytohormones play a central role in plant physiology. Despite the significant understanding in the hormone signaling, a deep understanding of downstream targets is missing. Therefore, we used a shotgun proteomics approach to investigate the ethylene, ABA and ABA+ethylene signaling in soybean leaves. A protamine sulfate precipitation (PSP) method was used for the enrichment of the low-abundance proteins which were then identified by label-free quantitative proteomics. A total of 5171 unique proteins were identified, of which 1182 were differentially modulated in one or more treatments. Functional annotation of the differentially modulated proteins showed that these were mainly associated with the biosynthesis of secondary metabolites and fatty acid metabolism.