PHRF1 promotes the class switch recombination of IgA in CH12F3-2A cells-Dataset

 PHRF1 is an E3 ligase that promotes TGF-B signaling by ubiquitinating a homeodomain repressor TG-interacting factor (TGIF). The suppression of PHRF1 activity by PML-RAR facilitates the progression of acute promyelocytic leukemia (APL). PHRF1 also contributes to non-homologous end-joining in response to DNA damage by linking H3K36me3 and NBS1 with DNA repair machinery. However, its role in class switch recombination (CSR) is not well understood. In this study, we report the importance of PHRF1 in IgA switching in CH12F3-2A cells and CD19-Cre mice. Our studies revealed that Crispr-Cas9 mediated PHRF1 knockout and shRNA-silenced CH12F3-2A cells reduced IgA production, as well as decreased the amounts of PARP1, NELF-A, and NELF-D. The introduction of PARP1 could partially restore IgA production in PHRF1 knockout cells. Intriguingly, IgA, as well as IgG1, IgG2a, and IgG3, switchings were not significantly decreased in PHRF1 deficient splenic B lymphocytes isolated from CD19-Cre mice. The le..., The file is the result of RNA-seq analysis in \"PHRF1 promotes the class switch recombination of IgA in CH12F3-2A cells\". The samples include normal CH12F3-2A cells and PHRF1 gene knockout CH12F3-2A cells. RNA was extracted and sequenced by next-generation sequence. The raw data is analyzed with KEGG pathway enricment., The file is compatible with Microsoft Excel., # Title of Dataset:

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PHRF1 promotes the class switch recombination of IgA in CH12F3-2A cells-Dataset

## Description of the Data and file structure

The file is the result of RNA-seq analysis in \"PHRF1 promotes the class switch recombination of IgA in CH12F3-2A cells\". The samples include normal CH12F3-2A cells and PHRF1 gene knockout CH12F3-2A cells. RNA was extracted and sequenced by next-generation sequence. The raw data is analyzed with the bioinformatic tool.

File Cr\_wt\_Comparison\_CH12F3\_FC\_over\_2 is RNA seq data from 3 normal CH12F3-2A cells (WT) and 3 PHRF1 gene knockout CH12F3-2A cells (Cr). The raw data is analyzed with CLC Genomics Workbench v10. The comparison report contains the following information:
Name: Gene name.
Chromosome: Chromosome of gene
Region: Region of gene
Gene length: The length of gene.
Exon length: The total length of exon.
Exon: The number of exon.
Gene ID: Gene ID
Gene Description: Description of gene
EntrezGene ID: The gene ID in NCBI
Biotype...