G-quadruplex in the gene of the large subunit of plant RNA polymerase II: billion years old story

Supplementary material to the journal article: Consist of: Difference CD spectra - comparison without and with previous UV treatment (Difference CD spectra - comparison without and with previous UV treatment.pdf) 40 bp long potential G4 locus is the most conserved site in the whole ~ 6000 bp long <em>rpb1</em> gene. See the histogram below the alignment: the position of the G4 locus is depicted, together with the horizontal red dashed line indicating relative nucleotide conservation among aligned sequences of<em> rpb1</em> (G4 locus is the most conserved within rpb1 gene.PNG) Prediction of G4-forming potential of inspected <em>rpb1</em> locus(G4 sequences and scores.xlsx) Graphical abstract (Graphical abstract.PNG) PDB structure of modeled G-quadruplex structure (Modelled 3D structure of G4 from Bathycoccus prasinos.tar.gz) Multiple alignment of <em>rpb1</em> paralogs (<em>rpa1</em>, <em>rpc1</em>, <em>rpd1a</em>, <em>rpd1b</em>) in <em>Arabidopsis thaliana </em>centered to G4 locus of <em>rpb1 </em>(Multiple_alignment_nrpsG4_arabidopsis.png) Multiple sequence alignment (using MUSCLE algorithm) of <em>rpb1</em> CDS sequences (rpb1_aligned_sequences_all.txt) All 20 <em>rpb1</em> CDS sequences in FASTA format (rpb1_sequences_all.txt) Five paralogs from <em>Arabidopsis thaliana</em> (<em>rpa1</em>,<em>rpb1</em>, <em>rpc1</em>, <em>rpd1a</em>, <em>rpd1b</em>) in FASTA format (rpbA_B_C_D sequences arabidopsis.txt) Gel electrophoresis and ThT staining of the selected G4-forming sequences