Methylation and gene expression data obtained from Dnmt3b+/+, Dnmt3bD/D and Dnmt3bCI/CI MYC induced T-cell lymphomas (MTCLs).

DNA methylation has suppressive effects on gene transcription and it is involved in a variety of physiologic processes, including development and cancer. As we and others demonstrated, Dnmt3b is a tumor suppressor in oncogene-driven lymphoid and myeloid malignancies in mice. Due to numerous activities such as methylation-dependent and independent repression and accessory functions, it is difficult to pinpoint physiological processes solely dependent on catalytic activity of Dnmt3b. By utilizing our new mouse model expressing catalytically inactive Dnmt3b at physiological levels we identified genome-wide methylation changes and aberrant gene expression profiles that are specific to catalytic activity of DNMT3b. Overall design: We performed WGBS and RNA-seq analysis on Dnmt3b+/+, Dnmt3bD/D and Dnmt3bCI/CI MYC-induced T-cell lymphoma samples isolated from lymph nodes of terminally sick mice. WGBS experiments were performed in biological duplicates, RNA-seq experiments in triplicates. In addition we performed RNA-seq on human peripheral T-cell lymphomas (hPTCLs) and normal human CD8+ and CD4+ T-cells.