Allele-specific expression analysis of Masculinizer (Masc) gene knocked-down embryos in the silkworm, Bombyx mori

The goal of this study is to know whether one of the two Z chromosomes is inactivated or both Z chromosomes are suppressed in Bombyx mori males. Transcriptome analysis was performed using Masc knocked-down hybrid embryos between two B. mori strains. The obtained polymorphic information can be used for allele-specific expression analysis.