Construction and Synchronization of dnaA Temperature-Sensitive Mutants of Streptomyces
收藏PubMed Central2026-05-16 收录
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https://pmc.ncbi.nlm.nih.gov/articles/PMC134811/
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资源简介：
Temperature-sensitive mutants of Streptomyces defective in initiation of chromosome replication were created by in vitro site-directed mutagenesis in the dnaA gene followed by gene replacement. When they were shifted to 39°C replication in the mutants ceased in about 90 min but resumed on return to 30°C. This allowed manipulations to achieve replication synchronization.
提供机构：
American Society for Microbiology (ASM)
