The Role of CD83 in ovarian cancer cell line SKOV3

We performed RNA-Seq of CD83 overexpression SKOV3 cells (OV_1, OV_2, OV_3), CD83 knockdown SKOV3 cells (KD_1, KD_2, KD-3), and control SKOV3 cells (NC_1, NC_2, NC_3) using BGISEQ-500 platform (BGI, China). Overall design: To initiate this study, we developed human CD83 stable overexpression and knockdown sublines of SKOV3 cells, using infection of full length CD83 lentivirus (CD83-OV) and CD83-specific lentiviral shRNA (CD83-KD), respectively. LV5 or LV3 lentivirus, containing GFP and puromycin resistant site, was served as negative control (NC). Polybrene (4 µg/ml) was used to increase the lentivirus infection efficiency. GFP-positive stable transfected cell lines were generated by two rounds of puromycin (1µg/ml) selection.