Increased production of TAPP induced by hypoxia as determined by a quantitative analysis using the Bio-Rad Experion.

Human glioma cells (U251, T98G, LNZ308 and D54) were cultured for 1 week either under hypoxic or normoxic conditions. The cells were harvested, lysed and the protein concentration was determined by Lowry method. One µg/µl of total protein were run on the Pro260 protein chips and then quantitated. Recombinant EZH2, Whsc2 and YKL-40 were purchased from US Biologicals and used as the standard to calculate true protein concentrations.