Anti-lipopolysaccharide antibody ruminal administration mitigates the ruminal lipopolysaccharide release without acute phase inflammatory and hepatic transcriptomic responses during the subacute ruminal acidosis challenge in Holstein bull cattle

The effects of anti-lipopolysaccharide (LPS) antibody on ruminal fermentation, LPS activity, and liver transcriptomes were investigated during the subacute ruminal acidosis (SARA) challenge. Eleven fistulated Holstein bull cattle (164 ± 14 kg; 5–6 months of age) were used in a replicated 3×3 Latin square design. Cattle were fed a roughage (orchard and timothy mixed hay) diet during the first 11 days (days -11 to -1; pre-challenge), a high-grain (50% concentrate and 50% soybean flakes) diet for 2 days (days 0 and 1; SARA-challenge), and then a roughage diet for 1 day (day 2; post-challenge) (Table 1). The cattle were administered with 0 (control group), 2, or 4 g immunoglobulin yolk containing anti-LPS antibody per head once daily through the rumen fistula for 14 consecutive days.