Proper splicing contributes to visual function in the aging Drosophila eye - Supporting data for Stegeman et al. (2018)

<p>Splicing was analyzed in the transcriptome of aging <em>Drosophila melanogaster</em> photoreceptors and eyes using RNA-seq. This dataset contains the original microscopy images (tif files), summaries of qPCR, phototaxis and luciferase assay data (csv files) used to generate the figures presented in Stegeman et al. 2018. It also contains R scripts used for analysis, plasmid map and sequence information for the Rh1-ffluc construct, and detailed protocol files for phototaxis assays, optical neutralization and luciferase assays. JunctionSeq plots for splicing analysis in photoreceptors and eyes are provided as zipped files that contain html files of all differentially-spliced genes. These plots show each splicing event (eg exon skipping/inclusion) normalized to overall gene expression using several different approaches (eg counts, normalized transcript levels).</p>