Oligonucleotides used in the different real-time LCR trials.
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The nucleotides which complement to the mutant SNP target are underlined on the discriminating oligonucleotides.aThe melting temperature Tm was estimated using the DINAMelt web server (http://www.bioinfo.rpi.edu/applications/hybrid/hybrid2.php).b5′ phosphorylation on the ligating oligonucleotide.
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2015-12-02



