Expanded phylogeny of Nomadinae (Hymenoptera: Apidae) with integration of UCE and DNA barcode sequence data

The subfamily Nomadinae within the family Apidae is the oldest and most diverse clade of brood parasitic bees. Through the incorporation of data from a variety of sources, we generated the most detailed and taxonomically complete phylogeny of this group to date. Despite differing amounts of genetic data available for different species, the topology of the tree broadly agrees with expected relationships based on previous findings, and all major groups were recovered as monophyletic. We further carried out divergence time estimation to investigate the evolutionary history of Nomadinae and place the phylogeny along the geological time scale, recovering an estimated age of 99 Ma for the group. Testing for the effect of barcode-only taxa on estimated dates indicated that ages for deep nodes were robust, though the inclusion of such taxa with limited sequence data tended to push shallower nodes towards older dates. The successful integration of cytochrome oxidase DNA barcode sequences with mo..., , , # Expanded phylogeny of Nomadinae (Hymenoptera: Apidae) with integration of UCE and DNA barcode sequence data

[https://doi.org/10.5061/dryad.12jm63z5d](https://doi.org/10.5061/dryad.12jm63z5d)

**1. 415alltaxa_1245loci+COI_matrix.fasta**

Alignment file including all 415 taxa (backbone + barcode), all 1245 UCE loci, and COI barcode sequence

**2. 228ucetaxa_1245loci_noCOI_matrix.fasta**

Alignment file including 228 UCE backbone taxa and all 1245 UCE loci

**3. 415alltaxa_50BPloci+COI_matrix.fasta**

Alignment file including all 415 taxa (backbone + barcode), and top 50 UCE loci by bipartition support

**4. 415alltaxa+1245loci+COI_SWSCpartitioned.treefile**

Main phylogeny including all 415 taxa (backbone + barcode), based on SWSC-EN partiioned alignment of all 1245 UCE loci and COI barcode sequence (with merged partitions)

**5. 228ucetaxa_1245loci_noCOI_SWSCpartitioned.treefile**

Phylogeny including 228 UCE backbone taxa, based on SWSC-EN partiioned alignment of all 1245 UCE loci (w...,