EC50 values of Prestwick Library hit compounds on the different cell types tested.

Screening of the Prestwick Library on TG1 human glioblastoma stem-like cells (GSCs) unveiled 20 molecules decreasing the relative ATP level in these cells. Dose response curves were performed with the 20 molecules using ATP Glo assay as readout. Molecules were tested on 3 different types of proliferating (P) and quiescent (Q) GSCs (TG1, TG16, OB1), non-cancer neural cells including human fetal neural stem cells (f-NSC) and primary astrocytes (HA cells) as well as HEK 293 cells, a non-cancer and non-neural cell line. Hits are classified into 3 groups according to their differential activity or not on proliferative and quiescent TG1 GSCs. In each group, molecules were clustered according to their ATC (Anatomical, Therapeutic, Clinical) classification. nd: not determined.
*: the maximum effect observed for this compound on HA cells was a reduction of only 30% of ATP levels which was not due to cell death.
EC50 values of Prestwick Library hit compounds on the different cell types tested.