Comparison of untreated and vancomycin-treated macrophages, with or without Candida albicans infection

Vancomycin is a widely prescribed antibiotic used in the treatment of Gram-positive bacterial infections. We recently showed that this antibiotic disrupted protective anti-fungal immune responses via microbiome dysbiosis, enhancing susceptibility to invasive candidiasis. Antibiotics are an independent risk factor for developing this life-threatening fungal infection, but whether microbiota-independent mechanisms also drive this association is not clear. Here, we have compared transcriptional responses of mouse bone-marrow derived macrophages at an early time point post-infection (2 hours), comparing back to baseline, using untreated or vancomycin-treated macrophages. Mouse bone-marrow derived macrophages were differentiated in presence of vancomycin or in antibiotic-free conditions. Macrophages were then left uninfected or infected with live Candida albicans yeast (strain SC5413) for two hours. RNA was isolated from all conditions using the Trizol method.