Molecular basis of GA-independent DELLA regulation by the VIH-type inositol pyrophosphate synthase in a non-vascular land plant

To study the functional relationship between MpVIH and MpDELLA at molecular level, the system-wide transcriptome profile of the following genotype were analysed: WT, CRISPR/Cas9 knockout lines of MpVIH (Mpvih) and independent Mpvih transgenic lines with reduced MpDELLA transcripts using an artificial microRNA (Mpvih::amiR-MpDELLA) technology. Overall design: Transcriptome comparison between WT, Mpvih mutant and amiR-MpDELLA lines in Mpvih background. For each genotype (Mpvih and Mpvih::amiR-MpDELLA) two independent lines were analysed. Each genotype has 3 biological replicates.