Short-term temporal variation of coastal marine eDNA

Temporal variation in eDNA signals is increasingly explored for understanding community ecology in aquatic habitats. Seasonal changes have been addressed using eDNA sampling, but very little is known regarding short-term temporal variation that spans hours to days. To address this, we filtered marine water samples from a single coastal site in Denmark every hour for 32 h. We used metabarcoding to target both fish and broader eukaryote diversity and evaluated temporal changes in this marine community. Results revealed variation in fish species richness (15–27) and eukaryote class richness (35–64) across the 32 h of sampling, and we further evaluated sampling efforts needed to reach different levels of diversity saturation. Relative read frequency data for both fish and eukaryotes indicated a clear diel change in community composition, with different communities detected during daylight versus dark hours. The abundance signals in our data reflected biological variation rather than stochas..., This dataset represents environmental DNA sequencing data from Skovshoved Harbour (Denmark) collected on 11th-12th of September, 2017. Samples were collected every hour for 32 hours straight, starting from 10 AM on the 11th and until 5 PM on the 12th (see connected publication for additional details).
DNA has been amplified with two different primer sets, namely the MiFish (for 12S fish data) and the 18S_allshorts (for 18S eukaryote data) primers. MiFish primers consists of the forward primer MiFish-U-F (5′-GTCGGTAAAACTCGTGCCAGC-3′) and the reverse primer MiFish-U-R (3′-GTTTGACCCTAATCTATGGGGTGATAC-5′), targeting a 163-185 bp fragment of 12S. Eukaryote amplification was done with forward primer 18S_allshorts forward (5’-TTTGTCTGSTTAATTSCG-3’) and reverse primer 18S_allshorts reverse (5’-CACAGACCTGTTATTGC-3’), targeting ca. 110 bp of 18S. The libraries have been sequenced using paired end NovaSeq 6000 sequencing (150 BP PE).
Libraries are named M1-M4 for the fish data and M1_KBJ-M4_KBJ fo..., We suggest you put the files in 8 separate folders in order to demultiplex. The files should be placed accordingly:
Folder 1: MiFish PCR replicate one
M1_MD5.txt (to check sums)
M1_UKKD19030017_HFCCTDSXX_L4_1.fq.gz
M1_UKKD19030017_HFCCTDSXX_L4_2.fq.gz
M1_12S_tags_1.list
Folder 2: MiFish PCR replicate two
M2_MD5.txt (to check sums)
M2_UKKD19030018_HF7V2DSXX_L3_1.fq.gz
M2_UKKD19030018_HF7V2DSXX_L3_2.fq.gz
M2_12S_tags_2.list
Folder 3: MiFish PCR replicate three
M3_MD5.txt (to check sums)
M3_UKKD19030019_HFCG7DSXX_L1_1.fq.gz
M3_UKKD19030019_HFCG7DSXX_L1_2.fq.gz
M3_12S_tags_3.list
Folder 4: MiFish PCR replicate four
M4_MD5.txt (to check sums)
M4_UKKD19030020_HF7V2DSXX_L1_1.fq.gz
M4_UKKD19030020_HF7V2DSXX_L1_2.fq.gz
M4_12S_tags_4.list
Folder 5: 18S_allshorts (eukaryote) PCR replicate one
M1_KBJ_MD5.txt (to check sums)
M1_KBJ_FKDL190745191-1a_H2WGFCCX2_L4_1.fq.gz
M1_KBJ_FKDL190745191-1a_H2WGFCCX2_L4_2.fq.gz
M1_KBJ_18S_tags_1.list
Folder 6: 18S_allshorts (eukaryote) PCR replicate two
M2_KBJ_MD5...