ovarian granulosa cell metagenome

Sample Information Description:This metagenome dataset is derived from ovarian granulosa cells (GCs) collected from replacement gilts (Sus scrofa). All animals were raised under standard commercial farm conditions. The granulosa cell samples were aspirated from ovarian follicles at the expected puberty age (190 days), immediately snap-frozen in liquid nitrogen, and stored at -80C until RNA extraction. This sampling process ensures the integrity of the transcriptome for investigating the molecular response to dietary beta-carotene supplementation.Project Objective:This project employed RNA sequencing to investigate the transcriptomic alterations in ovarian granulosa cells following dietary beta-carotene supplementation. A total of 20 healthy replacement gilts at 130 days of age were randomly assigned to two groups: a control group fed a basal diet and a treatment group supplemented with 10 mg/kg beta-carotene for 60 days. Granulosa cell samples were collected from 4 biologically independent replicates per group at slaughter.Strand-specific RNA-seq libraries were constructed and sequenced on the Illumina NovaSeq 6000 platform. Differential expression analysis identified a set of significantly dysregulated genes. Notably, the upregulation of key transcripts included FOXL2 and SOX12 (critical for ovarian development); OSR1 (involved in steroidogenesis); PAPPA (a key IGFBP protease); ALAS1 and FDX1 (central to heme synthesis and steroidogenesis). Conversely, downregulated genes such as SEMA6A, TMEM100, and WNTSA suggest potential modulations in cell signaling.This dataset provides a comprehensive resource for understanding the molecular mechanisms by which dietary beta-carotene influences key ovarian processes, including steroidogenesis, redox balance, and granulosa cell differentiation.