Single Cell Profiling Identifies the Histone Demethylase, JMJD3, Modulates Macrophage Phenotype in Abdominal Aortic Aneurysm Formation. Single Cell Profiling Identifies the Histone Demethylase, JMJD3, Modulates Macrophage Phenotype in Abdominal Aortic Aneurysm Formation

Abdominal aortic aneurysm (AAA) is a life-threatening disease characterized by an inflammatory macrophage phenotype that contributes to pathological vascular remodeling. Although the mechanisms regulating macrophage-mediated inflammation during AAA development remain undefined, accumulating evidence suggests that epigenetic alterations play a critical role. Here using single cell RNA-sequencing techniques, we identify that the histone demethylase JMJD3 is elevated in human AAA monocytes/macrophages, resulting in the upregulation of cellular activation and a proinflammatory immune response. Overall design: scRNA-seq for AAA