Summary of clinical data and Treg-mediated suppression.

A summary of clinical parameters of all HIV+ subjects studied along with the following functional data is summarised: (i) Absolute CD4+CD25+FoxP3+ Treg cell number and % specific Treg-mediated suppression of (ii) effector cell proliferation or (iii) single IFN-gamma expression. Effector cells in both suppression assays were stimulated with 2∶1 CD3/28 bead to cell ratio. E∶T ratio was 1∶0.125 in proliferation assay and 1∶0.001 in the IFN-gamma suppression assay. Asterik denotes HIV+ subjects who showed significantly elevated suppression compared to the mean suppression level noted in control subjects under identical culture conditions in both suppression assay. For age and gender of controls, refer to materials and methods.