gene expression in hippocampus from ophn1tm1Bill mouse males and controls during 24h

Oligophrenin1 X-linked Intellectual disability (ID) gene is mutated in ID patients with cerebellar hypoplasia. It encodes OPHN1 protein which has been shown to interact with Rev-erb alpha, a negative regulator of the circadian clock in cells (https://doi.org/10.1038/nn.2911) In this study, we searched for gene expression differences in hippocampi from adult mouse ophn1 KO and controls during 24 hours. Three replicates were collected every 6 hours for both genotypes. Data from controls were analyzed and reported in http://dx.doi.org/10.1016/j.neuroscience.2015.08.066. Adult males were placed in dark room for 24 hours before the collection of the hippocampus every 6 hours . PCA allowed the exclusion of two time points in controls (t=0 and 12).X连锁智力障碍（intellectual disability, ID）基因Oligophrenin1在伴有小脑发育不全的智力障碍患者中发生突变。该基因编码OPHN1蛋白，已有研究证实该蛋白可与细胞生物钟负调控因子Rev-erbα相互作用（https://doi.org/10.1038/nn.2911）。本研究旨在检测成年小鼠ophn1基因敲除（knockout, KO）组与对照组的海马组织在24小时周期内的基因表达差异。两种基因型小鼠均每6小时采集3份生物学重复样本。对照组的相关数据分析结果已发表于文献http://dx.doi.org/10.1016/j.neuroscience.2015.08.066。本实验采用成年雄性小鼠，在每6小时一次的海马组织采集前，需将小鼠置于暗室适应24小时。通过主成分分析（principal component analysis, PCA），我们剔除了对照组的两个时间点样本（t=0与t=12）。