Immune Education Promotes T Cell Survival in Mice Subjected to the Cecal Ligation and Puncture Sepsis Model

This study utilized anti-CD3e antibody to induce T cell memory (a process herein defined as Immune Education) to address the lack of diverse memory T cell compartments in lab mice, which limits the fidelity of the cecal ligation and puncture (CLP) as a model of human sepsis. However, induced T cell memory via Immune Education necessitates examination of the effect of CLP on specific T cell subsets that were previously poorly represented in lab mice. Using single-cell RNA sequencing (scRNA-seq), we defined T cell subset responses to CLP, and specifically interrogated the effects of Immune Education on these responses.Splenic T cells were isolated from C57BL/6 mice. The four cohorts for the scRNA-seq experiment were Control, Immune-Educated, CLP, and Immune-Educated CLP. The Immune-Educated and Immune-Educated CLP mice underwent Immune Education at 8 weeks of life using anti-CD3e antibody while the Control and CLP mice were administered an isotype control. CLP was performed using 22-gauge double puncture at 12-13 weeks of life. Mice were sacrificed at baseline or 24-hours post-CLP.