RNA-sequencing of brain mural cells differentiated from human pluripotent stem cell-derived neural crest via Notch3 activation

Molecular mechanisms underlying the differentiation of brain mural cells from neural crest are poorly understood. We found that activation of Notch3 signaling in human pluripotent stem cell-derived neural crest (using lentiviral overexpression of the human Notch3 intracellular domain, N3ICD) was sufficient to direct the differentiation of mural cells. RNA-seq was performed on cells from the DF19-9-11T, H9, IMR90-4, and WTC11 hPSC lines. For each line, differentiation-matched samples of neural crest cells, and GFP-negative and GFP-positive cells isolated via FACS 6 days after transduction of neural crest with N3ICD-GFP lentivirus, were analyzed.