EXTRAMETABOLOME ANALYSIS OF SALMONELLA corC MUTANTS BY NMR

Description

Magnesium is an essential divalent metal that serves many cellular functions in growing cells. In Salmonella typhimurium, recent data from our collaborators of the PERIOMET ANR project showed the importance of magnesium in the ability of quiescent Salmonella cells to resume growth (Metaane et al. 2022 & 2023). During the study of magnesium and cobalt transport by CorA in Salmonella, three loci named corB, corC and corD, involved in resistance to cobalt and magnesium efflux have been discovered by Gibson et al. (1991). It was proposed that CorB, CorC and CorD contribute to the efflux of divalent cations by CorA, either by coupling the transport of cations by CorA to an energy source, or as a cation sensor for the CorA protein. The corB, corC and corD genes have not been further characterized in Salmonella and their function remains unclear to date. The corB, corC and corD genes correspond to the yfjD, ybeX and possibly apaG genes, respectively, in Salmonella serovar Typhimurium strain ATCC14028. Our collaborators have constructed deletions in these genes in order to study their physiological impact in Salmonella. 

In the present study, we report the extrametabolome analysis by NMR of the WT and corC mutant in quiescent Salmonella cells after culture in presence of different magnesium concentrations.

In other related reports: 

Description of the construction of corC, corB and corD mutants and their resistance phenotype towards cobalt, nickel and magnesium toxicity. NOREL Françoise, MONTEIL Véronique, & METAANE Selma. (2023). Towards new elements involved in magnesium and cobalt trafficking in Salmonella serovar Typhimurium. Zenodo. https://doi.org/10.5281/zenodo.8086417

The bacterial two hybrid system (BACTH) was used to find out potential physical interactions between CorA, CorB(YfjD), CorC(YbeX) and ApaG.  NOREL F, METAANE S, & MONTEIL V. (2023). Detection of physical interactions between the magnesium transporter CorA and other Cor proteins using the bacterial two hybrid system (BACTH). Zenodo. https://doi.org/10.5281/zenodo.7994619

Mass spectrometry proteomics was used to assess global effects of deletion of the corC gene on the Salmonella proteome. The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD043903.

This work was supported by the French National Research Agency (ANR-19-CE44-0005-01, PERIOMET project).

References

Gibson MM, Bagga DA, Miller CG, Maguire ME. Magnesium transport in Salmonella typhimurium: the influence of new mutations conferring Co2+ resistance on the CorA Mg2+ transport system. Molecular microbiology. 1991;5(11):2753-62.

Metaane S, Monteil V, Ayrault S, Bordier L, Levi-Meyreuis C, Norel F. The stress sigma factor sigmaS/RpoS counteracts Fur repression of genes involved in iron and manganese metabolism and modulates the ionome of Salmonella enterica serovar Typhimurium. PloS one. 2022;17(3):e0265511.

Metaane S, Monteil V, Douché T, Giai Gianetto Q, Matondo M, Maufrais C, Norel F. Loss of CorA, the primary magnesium transporter of Salmonella, is alleviated by MgtA and PhoP-dependent compensatory mechanisms. PloS one. 2023;18(9):e0291736.