Data results of glyphosate

These figures are results of search treatment mouse with glyphosate. The present study aimed at assessing the induction of mutagenic changes and oxidative stress in different tissues of animals exposed to glyphosate and Trop®. Male Swiss mice were submitted to gavage treatment with glyphosate and Trop® at the concentrations of 50 and 200 mg/kg for 5 days. After the treatments the animals were euthanized and their bone marrow, liver, lung, and kidney were collected. The bone marrow was used for micronucleus. The liver, lung and kidney were submitted to biochemical analyzes of thiobarbituric acid reactive substances (TBARS), catalase enzyme, total antioxidant, in addition to evaluating the expression of Bax, Bcl2 and p53 proteins, as well as the expression of p53 gene transcription. The animals were weighed before and after the treatments. The results showed a significant weight reduction in the animals treated for 5 days. The occurrence of micronuclei was significant in the bone marrow. A significant increase in lipid peroxidation, besides changes in both, the catalase enzyme and total antioxidant levels were seen in all tissues. There was a decrease in p53 gene and Bax protein expression, and an increase of the Bcl2 protein expression. These results might suggest that the exposure to glyphosate and the consumption of antioxidant defenses can cause oxidative stress. In addition, changes in cellular biomarkers regarding glyphosate and Trop® were similar.

本研究旨在评估动物暴露于草甘膦和Trop®后，不同组织中突变性变化和氧化应激的诱导。雄性瑞士小鼠被给予50mg/kg和200mg/kg浓度的草甘膦和Trop®进行灌胃治疗，连续5天。治疗后，动物被安乐死，其骨髓、肝脏、肺和肾脏被收集。骨髓用于微核测试。肝脏、肺和肾脏进行了硫代巴比妥酸反应物质（TBARS）、过氧化氢酶酶、总抗氧化剂等生化分析，并评估了Bax、Bcl2和p53蛋白的表达，以及p53基因的转录表达。动物在治疗前后均进行了称重。结果显示，连续治疗5天的动物体重显著下降。骨髓中微核的发生率显著。所有组织中均观察到脂质过氧化显著增加，同时过氧化氢酶酶和总抗氧化剂水平也发生了变化。p53基因和Bax蛋白表达减少，Bcl2蛋白表达增加。这些结果可能表明，草甘膦的暴露和抗氧化防御机制的消耗可能导致氧化应激。此外，关于草甘膦和Trop®的细胞生物标志物变化相似。