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FRET data for manuscript "Light-Induced Triplet–Triplet Electron Resonance Spectroscopy with Orthogonal Chromophores allows Pulsed ESR Distance Measurements between FRET Labels"

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DataCite Commons2023-07-13 更新2025-04-17 收录
下载链接:
https://figshare.manchester.ac.uk/articles/dataset/FRET_data_for_manuscript_Light-Induced_Triplet_Triplet_Electron_Resonance_Spectroscopy_with_Orthogonal_Chromophores_allows_Pulsed_ESR_Distance_Measurements_between_FRET_Labels_/21961571
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资源简介:
Three FRET datasets for each of the samples/experiments measured in manuscript "Light-Induced Triplet-Triplet Electron Resonance Spectroscopy with Orthogonal Chromophores allows Pulsed ESR Distance Measurements between FRET Labels". Each dataset contains, in separate tabs: the UV/Vis absorbance spectrum of the acceptor+peptide molecule and calculated epsilon (spectrum normalised to molar extinction coefficient of acceptor), the raw and normalised fluorescence emission spectra of the donor+peptide molecule, the calculated overlap, and the raw fluorescence emission spectrum of the acceptor+peptide+donor molecule. A tab labelled values lists the values taken from literature to carry out calculations alongside values taken/calculated from the collected data. <br> In addition, datasets for the UV/Vis and fluorescence emission spectra used in determining the specific fluorescence quantum yield of <strong>[3]</strong> and <strong>[5]</strong> ("QYdetermination.xlsx") as well as steady state fluorescence emission anisotropy measurements for each of <strong>[1]</strong>-<strong>[5] </strong>("Anisotropy.xlsx"). <br> Experimental and computational methods are described in the SUPPORTING INFORMATION of the manuscript. DOI of the manuscript to be added after acceptance. A README is included to accompany datasets. Contents: EBdonorTPPacceptor.xlsx - dataset corresponding to FRET measurements of sample labelled <strong>[2] </strong>in manuscript. TPPacceptorZnTPPdonor(567ex).xlsx - dataset corresponding to FRET measurements of sample labelled <strong>[1] </strong>in manuscript, where 567 nm laser excitation was used: TPP was taken to be acceptor and ZnTPP was taken to be donor. TPPdonorZnTPPacceptor(510ex).xlsx - dataset corresponding to FRET measurements of sample labelled <strong>[1] </strong>in manuscript, where 510 nm laser excitation was used: TPP was taken to be donor and ZnTPP was taken to be acceptor. QYdetermination.xlsx - dataset corresponding to absorption and emission spectra used in determining the fluorescence quantum yield of <strong>[3]</strong> and <strong>[5]</strong>, using unbound TPP and rhodamine 6G respectively. Anisotropy.xlsx - dataset containing steady state fluorescence emission anisotropy measurements for each of compounds <strong>[1]</strong>-<strong>[5]</strong>. <br> <br>
提供机构:
University of Manchester
创建时间:
2023-03-20
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