PbAP2-FG2 and AP2R-2 function together as a transcriptional repressor complex essential for Plasmodium female development [ChIP-seq]

To investigate the genome-wide binding sites of the transcriptional regulators, PbAP2-FG2 and AP2R-2 in female gametocytes of Plasmodium berghei, the chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) analyses were performed. Parasites expressing GFP-fused PbAP2-FG2 (PbAP2-FG2::GFP) or GFP-fused AP2R-2 (AP2R-2::GFP) were harvested after treating the infected mice with sulfadiazine and subjected to ChIP-seq experiments using anti-GFP antibody. Binding sites of PbAP2-FG2 and AP2R-2 were determined from the sequence data. Mice were infected with PbAP2-FG2::GFP or AP2R-2::GFP and treated with sulfadiazine in their drinking water. Chromatins associated with GFP-tagged PbAP2-FG2 or AP2R-2 were IPed with anti-GFP antibody, and DNA fragments purified from the IPed chromoatins were sequenced by the Next Generation Sequencing. Using non-IPed samples as a control (INPUT), binding sites of PbAP2-FG2 and AP2R-2 were determined from the sequence data.