Sanger sequencing validation data for recombinant pPICZαA-elafin plasmid

This dataset contains the raw Sanger sequencing chromatogram and the annotated supplementary sequencing figure used to validate the recombinant pPICZαA-elafin plasmid described in the manuscript entitled “Secretory expression of recombinant elafin in Pichia pastoris reveals in vitro antibacterial and antifungal activity”. Sanger sequencing was performed by Macrogen, Inc. (Seoul, South Korea) using the AOX1 reverse primer. The sequencing read was analysed using SnapGene Viewer version 7.0.2 and aligned with the expected pPICZαA-elafin construct sequence. The dataset includes:1. ela_AOX1_Reverse.ab1 — raw Sanger sequencing chromatogram file.2. Figure S2.pdf — annotated Sanger sequencing chromatogram provided as Supplementary Figure S2.3. README.txt — description of the dataset and files. Sequencing was performed solely for plasmid construct verification, specifically to confirm the correct insertion and orientation of the elafin gene in the pPICZαA expression vector. This dataset does not represent de novo sequencing, genome sequencing, transcriptome sequencing, or novel DNA/RNA sequencing; therefore, no new GenBank accession number was generated.