Figure 1

Fig 1. HEK293T cells transduced with T7 RNAP support T7 promoter-dependent expression. HEK293T cells were transduced with MIP-T7RNAP or LPCX(AB)-T7RNAP or with the empty vectors as controls. Cells were treated with antibiotics to kill untransduced cells and then were transfected in triplicates with a plasmid expressing luciferase under control of the T7 promoter. Luciferase activity was quantified the next day as described in the Methods section. RLU, relative lights units. A Student’s t-test was used to assess statistical significance; **, P < 0.01; ***, P < 0.001.