Results of gene expression, WGCNA, and mapping bias analyses and body weight in RNAi strains.

A) Results of WGCNA and DESeq2 analyses for Dutch (NL) and Zambian (ZI) populations. Log2 fold change and adjusted P-values for within and between population comparisons for early (PS12), late (PS79) and prepupal (WPP) stages as well as adjusted P-values from likelihood ratio test (LRT) for an interaction between stage and population are shown. WGCNA module membership is also shown as well as total (kTotal) and within module (kWithin) connectivity. B) Results of DESeq2 analysis for the German (MU) population during the late stage. Log2 fold change and adjusted P-values are shown. C) Relative gene expression in TPM for Dutch and Zambian populations. D) Mapping bias in Dutch and Zambian populations. E) Body weight of groups of 5 flies in RNAi and control strains. (XLSX)