scRNA-seq transcriptomic profiles of cochlear and vestibular hair cells from adult mice [10_weeks_CBAJ]

The cochlear and vestibular sensory epithelia contain four different hair cell (HC) types for detecting sound and motion. There are substantial differences in morphology and function among them. We used single cell RNA sequencing (scRNA-seq) to analyze the diversity of HCs in the inner ear. Overall design: HCs from cochlear and vestibular sensory organs were isolated. cDNA library were constructed using the 10x Genomics Chromium Single Cell 3' Reagent Kits V3.1. 7 mice were used for each biological replicate, and 6 biological replicates for cochlear sensory epithelium and 4 biological replicates vestibular sensory epithelia were prepared. Illumina NextSeq 6000 sequencer was used for sequencing.