Autophosphorylation of insulin receptor

Receptor autophosphorylation requires a lysine at position 1057 to stabilize the gamma phosphate of ATP. The adenosine of ATP interacts with three glycines at residues 1030-1035. The first tyrosine residues to be autophosphorylated are 1185, 1189 and 1190 in the kinase domain. This is followed by 999 in the juxtamembrane domain, 1355 and 1361. These tyrosines fall into the three distinct tyrosine phosphorylation domains of the beta-subunit. In total there are 13 potential tyrosines that may be phosphorylated. The receptor phosphorylates itself in a trans rather than cis manner, where the beta-subunits phosphorylate each other rather than themselves (Tavaré et al. 1998).

受体自磷酸化过程依赖于位于1057位的赖氨酸以稳定ATP的γ磷酸基团。ATP中的腺苷与1030-1035位残基上的三个甘氨酸相互作用。首先被自磷酸化的酪氨酸残基位于激酶域的1185、1189和1190位。随后是紧邻膜域的999位，以及1355和1361位。这些酪氨酸归属于β亚基的三个不同的酪氨酸磷酸化域。总计存在13个潜在的酪氨酸磷酸化位点。该受体以反式而非顺式的方式实现自我磷酸化，即β亚基相互磷酸化而非自我磷酸化（Tavaré等，1998年）。