DHT alleviates neuroinflammation to reduce PND occurrence in aged mice via mmu_circ_0001442/miR-125a-3p/NUFIP2 axis

To clarify the role of circRNA in DHT improving cognitive function in mice with perioperative neurocognitive disorder, circRNA sequencing was preformed to analyze the expression of circRNA in hippocampus of mice. Overall design: The hippocampus tissue of representative mice was extracted for circRNA sequencing in the testosterone group and the control group. Gene abundance was calculated and normalized by the SRPBM (spliced reads per billion mapping) method. The DESeq2 (V1.6.3) of the Bioconductor software package was used to identify differentially expressed circRNA between DHT-treated and control groups. Significantly different genes were those with (FC) = 2 and P value < 0.05.