small RNA-seq of ICR mouse sperm with different combinations of in utero undernutrition and post natal undernutrition

Maternal caloric restriction during ?the last week of gestation resulted in a low birth weight (LBW) and increased risk of LBW-associated metabolic diseases in adult life. The metabolic phenotypes transmitted to F2 generation by paternal manner without additional altered nutrition. To investigate the mechanism of this intergenerational inheritance, two Cohorts were exposed to different magnitudes of unternourition both in utero during the last week of gestation and/or post nataly until weaning. We performed small RNA-seq on non-coding RNA from sperm collected from these mice. Small RNA libraries were size-selected to be between 132 to 200 bp (including adapter) after library amplification.