Determination of the specificity profiling of native and synthetic cardosin B using proteomic identification of protease cleavage sites (PICS)

We performed an analysis of the substrate specificity preferences of native and synthetic cardosin B using the Proteomic Identification of protease Cleavage Sites methodology and some differences regarding preference and stringency of the subsite binding pockets were observed. Results clearly suggest that synthetic cardosin B is more stringent than native cardosin B explaining the observed suitability of the synthetic protein as biotechnological tool in cheese industry.