ANDe™ : a fully integrated environmental DNA sampling system

1. Species monitoring from environmental DNA (eDNA) is a powerful new technique for natural resource scientists and the number of research groups employing eDNA detection is growing rapidly. However, current eDNA sampling technologies consist mainly of do-it-yourself solutions, and the lack of purpose-built sampling equipment is limiting the efficiency and standardization of eDNA studies.
2. Here, we describe the first fully-integrated sampling system (ANDeTM) designed by a team of molecular ecologists and engineers for high-throughput eDNA sample collection. It consists of a backpack portable pump that integrates sensor feedback, a pole extension with remote pump controller, custom-made filter housings in single-use packets for each sampling site, and onboard sample storage. The system is optimized for sampling speed and replicability, while minimizing risk of contamination.
3. We present an example pilot study designed to identify optimal ANDeTM system parameter values (i.e., pump p...